Journal: Frontiers in Medicine
Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction
doi: 10.3389/fmed.2025.1653708
Figure Lengend Snippet: The binding characteristic of AXL-4#. (A) Representation of interplay of AXL antibody and Gas6. (B) 293T-hAXL cells were incubated with isotype control (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (C) 293T-memGas6 cells were incubated with anti-Gas6l (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (D,E) The structure of αAXL-4# recognizing Gas6/AXL complex predicted by AlphaFold.
Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.
Techniques: Binding Assay, Incubation, Control, Staining